JAK2 antibody (C-Term)

Catalog No. ABIN6992101

This Rabbit Polyclonal antibody specifically detects JAK2 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for JAK2 antibody (C-Term) (ABIN6992101)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JAK2 Antibody

Binding Specificity: C-Term

Specificity: JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.

Purification: JAK2 antibody is affinity chromatography purified via peptide column.

Immunogen: JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2. The immunogen is located within the last 50 amino acids of JAK2.

Isotype: IgG

Application Details

Application Notes: JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: JAK2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: JAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).

Molecular Weight: Predicted: 125 kDa

Observed: 125 kDa

Gene ID: 3717

NCBI Accession: NP_004963

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response