MEK1/2 antibody (AA 240-290)

Catalog No. ABIN6992146

This Rabbit Polyclonal antibody specifically detects MEK1/2 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MEK1/2 antibody (AA 240-290) (ABIN6992146)

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MEK1/2 Antibody

Binding Specificity: AA 240-290

Specificity: MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.

Purification: MEK1/2 antibody is affinity chromatography purified via peptide column.

Immunogen: MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1. The immunogen is located within amino acids 240 - 290 of MEK1/2.

Isotype: IgG

Application Details

Application Notes: MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MEK1/2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MEK1/2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MEK1/2

Background: The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals (2). MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2).

Molecular Weight: Predicted: 43 kDa

Observed: 44 kDa

Gene ID: 5604

NCBI Accession: NP_002746

UniProt: Q0VD16