HOOK3 antibody (N-Term)

Catalog No. ABIN6992177

The Rabbit Polyclonal anti-HOOK3 antibody is suitable to detect HOOK3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for HOOK3 antibody (N-Term) (ABIN6992177)

Target: HOOK3 (Hook Homolog 3 (HOOK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOOK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HOOK3 Antibody

Binding Specificity: AA 210-260, N-Term

Specificity: HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.

Purification: HOOK3 Antibody is affinity chromatography purified via peptide column.

Immunogen: HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3.

Isotype: IgG

Application Details

Application Notes: HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HOOK3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for HOOK3

Target: HOOK3 (Hook Homolog 3 (HOOK3))

Alternative Name: HOOK3

Background: The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems, their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).

Molecular Weight: Predicted: 79 kDa

Observed: 77 kDa

Gene ID: 84376

NCBI Accession: NP_115786

UniProt: Q86VS8

Pathways: Stem Cell Maintenance, Maintenance of Protein Location