alpha Tubulin antibody (C-Term) (Biotin)

Catalog No. ABIN6992214

This anti-alpha Tubulin antibody is a Rabbit Polyclonal antibody detecting alpha Tubulin in WB and ELISA. Suitable for Human, Mouse, Rat, Chicken, Zebrafish (Danio rerio) and Rabbit.

Quick Overview for alpha Tubulin antibody (C-Term) (Biotin) (ABIN6992214)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Chicken, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-alpha Tubulin Antibody (Biotin)

Binding Specificity: C-Term

Specificity: Biotin-Alpha-tubulin antibody is human, mouse, rat, rabbit, chicken, and zebrafish reactive.

Purification: Biotin-Alpha-tubulin antibody is affinity chromatography purified via peptide column.

Immunogen: Biotin-Alpha-tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-tubulin

Isotype: IgG

Application Details

Application Notes: Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Biotin-Alpha-tubulin antibody is supplied in PBS containing 1 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Biotin-Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha-tubulin

Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Molecular Weight: Predicted: 50 kDa

Observed: 55 kDa

Gene ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics