TIGIT antibody (Extracellular Domain)

Catalog No. ABIN6992247

The Mouse Monoclonal anti-TIGIT antibody (Clone 4A10) (ABIN6992247) specifically detects TIGIT in WB, ELISA, FACS, ICC, IHC (p) and IF. The antibody is reactive with Human samples.

Quick Overview for TIGIT antibody (Extracellular Domain) (ABIN6992247)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 4A10

Product Details anti-TIGIT Antibody

Binding Specificity: Extracellular Domain

Purification: TIGIT Antibody is supplied as protein A purified IgG1.

Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT

Isotype: IgG1

Application Details

Application Notes: TIGIT antibody can be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 1 μ,g/mL. For flow cytometry at 1 μ,g/mL. For immunocytochemistry at 1 μ,g/mL. For Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TIGIT Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TIGIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity, this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Molecular Weight: Predicted: 26 kDa

Observed: 47 kDa

Gene ID: 201633

NCBI Accession: NP_776160

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints