TIGIT antibody (AA 22-141)
-
- Target See all TIGIT Antibodies
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
-
Binding Specificity
- AA 22-141
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This TIGIT antibody is un-conjugated
-
Application
- ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Coating (Coat), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)
- Clone
- TIGIT-3017
- Isotype
- IgG2c kappa
- Top Product
- Discover our top product TIGIT Primary Antibody
-
-
- Application Notes
-
Positive Control: MOLT-4, Prostate or Colon Carcinoma.
Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Alternative Name
- TIGIT (TIGIT Products)
- Synonyms
- VSIG9 antibody, VSTM3 antibody, WUCAM antibody, ENSMUSG00000071552 antibody, Vstm3 antibody, RGD1563191 antibody, T-cell immunoreceptor with Ig and ITIM domains antibody, T cell immunoreceptor with Ig and ITIM domains antibody, TIGIT antibody, Tigit antibody
- Background
- TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.
- Molecular Weight
- 26kDa
- Gene ID
- 201633
- Pathways
- Cancer Immune Checkpoints
-