EPO antibody (AA 28-193)

Catalog No. ABIN7425611

This anti-EPO antibody is a Mouse Monoclonal antibody detecting EPO in WB and IHC. Suitable for Human.

Quick Overview for EPO antibody (AA 28-193) (ABIN7425611)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C3

Product Details anti-EPO Antibody

Binding Specificity: AA 28-193

Purpose: Monoclonal Antibody to Erythropoietin (EPO)

Specificity: The antibody is a mouse monoclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Erythropoietin (EPO) corresdonding to Ala28~Arg193 with N-terminal His and GST Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: Erythropoietin

Target Type: Hormone

Background: EP, Epoetin, Erythropoetin, Hematopoietin, Hemopoietin

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity