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IL-6 antibody (AA 30-208)

This Mouse Monoclonal antibody specifically detects IL-6 in WB, IHC, IP and ICC. It exhibits reactivity toward Sheep.
Catalog No. ABIN7426652

Quick Overview for IL-6 antibody (AA 30-208) (ABIN7426652)

Target

See all IL-6 (IL6) Antibodies
IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity

  • 208
  • 135
  • 76
  • 44
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  • 23
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  • 8
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  • 3
  • 2
Sheep

Host

  • 234
  • 146
  • 51
  • 14
  • 6
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  • 3
  • 2
Mouse

Clonality

  • 255
  • 201
  • 2
Monoclonal

Conjugate

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  • 3
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  • 3
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  • 2
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  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
This IL-6 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone

C1
  • Binding Specificity

    • 30
    • 29
    • 28
    • 15
    • 15
    • 15
    • 12
    • 10
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    • 6
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    • 5
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    • 3
    • 3
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-208

    Purpose

    Monoclonal Antibody to Interleukin 6 (IL6)

    Specificity

    The antibody is a mouse monoclonal antibody raised against IL6. It has been selected for its ability to recognize IL6 in immunohistochemical staining and western blotting.

    Purification

    Protein A + Protein G affinity chromatography

    Immunogen

    Recombinant Interleukin 6 (IL6) corresdonding to Gly30~Lys208 (Accession # P29455) with N-terminal His Tag

    Isotype

    IgG2b kappa
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    IL-6 (IL6) (Interleukin 6 (IL6))

    Alternative Name

    Interleukin 6

    Background

    MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor

    UniProt

    P29455

    Pathways

    TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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