Calnexin antibody (AA 239-461)

Catalog No. ABIN7425800

The Mouse Monoclonal anti-Calnexin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Calnexin in samples from Human.

Quick Overview for Calnexin antibody (AA 239-461) (ABIN7425800)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C8

Product Details anti-Calnexin Antibody

Binding Specificity: AA 239-461

Purpose: Monoclonal Antibody to Calnexin (CNX)

Specificity: The antibody is a mouse monoclonal antibody raised against CNX. It has been selected for its ability to recognize CNX in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Dog, Goat, Mouse, Pig, Rabbit, Rat, Sheep

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Calnexin (CNX) corresdonding to Thr239~Ala461 with N-terminal His and GST Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: CANX, CNX, IP90, P90, Major Histocompatibility Complex Class I Antigen-Binding Protein p88

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis