HMGB1 antibody (AA 1-215)

Catalog No. ABIN7428749

The Mouse Monoclonal anti-HMGB1 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect HMGB1 in samples from Human.

Quick Overview for HMGB1 antibody (AA 1-215) (ABIN7428749)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C1

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 1-215

Purpose: Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)

Specificity: The antibody is a mouse monoclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant High Mobility Group Protein 1 (HMGB1) corresdonding to Met1~Glu215 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome