Cyclin D1 antibody (AA 1-292)

Catalog No. ABIN7427000

The Mouse Monoclonal anti-Cyclin D1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Cyclin D1 in samples from Chicken.

Quick Overview for Cyclin D1 antibody (AA 1-292) (ABIN7427000)

Target: Cyclin D1 (CCND1)

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 5#

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 1-292

Purpose: Monoclonal Antibody to Cyclin D1 (CCND1)

Specificity: The antibody is a mouse monoclonal antibody raised against CCND1. It has been selected for its ability to recognize CCND1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cyclin D1 (CCND1) corresdonding to Met1~Ile292 (Accession # P55169)

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene

UniProt: P55169

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling