CD14 antibody (AA 20-368)

Catalog No. ABIN7425989

The Mouse Monoclonal anti-CD14 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CD14 in samples from Human.

Quick Overview for CD14 antibody (AA 20-368) (ABIN7425989)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C8

Product Details anti-CD14 Antibody

Binding Specificity: AA 20-368

Purpose: Monoclonal Antibody to Cluster Of Differentiation 14 (CD14)

Specificity: The antibody is a mouse monoclonal antibody raised against CD14. It has been selected for its ability to recognize CD14 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cluster Of Differentiation 14 (CD14) corresdonding to Thr20~Leu368 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades