AGT antibody (AA 25-477)

Catalog No. ABIN7426724

This Mouse Monoclonal antibody specifically detects AGT in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for AGT antibody (AA 25-477) (ABIN7426724)

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C17

Product Details anti-AGT Antibody

Binding Specificity: AA 25-477

Purpose: Monoclonal Antibody to Angiotensinogen (AGT)

Specificity: The antibody is a mouse monoclonal antibody raised against AGT. It has been selected for its ability to recognize AGT in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Angiotensinogen (AGT) corresdonding to Asp25~Val477 (Accession # P01015)

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for AGT

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Alternative Name: Angiotensinogen

Background: ANHU, SERPINA8, Angiotensin, Renin Substrate, Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

UniProt: P01015

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity