CRH antibody

Catalog No. ABIN7427969

Product Details anti-CRH Antibody

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Corticotropin Releasing Factor (CRF)

Specificity: The antibody is a mouse monoclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: OVA Conjugated Corticotropin Releasing Factor (CRF)

Clone: E1

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.64 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticotropin Releasing Factor (CRH Products)

Background: CRH, Corticotropin Releasing Hormone, Corticoliberin

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour