SMAD3 antibody (AA 231-425)

Catalog No. ABIN7426472

Product Details anti-SMAD3 Antibody

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Binding Specificity: AA 231-425

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Monoclonal Antibody to SMAD family member 3 (SMAD3)

Specificity: The antibody is a mouse monoclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant SMAD family member 3 (SMAD3) corresdonding to Phe231~Ser425 (Accession # P84022)

Clone: C4

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD family member 3 (SMAD3 Products)

Synonyms: HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

Background: JV15-2, MADH3, Mothers Against Decapentaplegic Homolog 3

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy