His Tag antibody

Catalog No. ABIN7426805

The Mouse Monoclonal anti-His Tag antibody has been validated for ELISA, IP, ICC, IHC (fro) and CLIA. It is suitable to detect His Tag in samples from Various Species.

Quick Overview for His Tag antibody (ABIN7426805)

Target: His Tag

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chemiluminescence Immunoassay (CLIA)

Clone: D10

Product Details anti-His Tag Antibody

Purpose: Monoclonal Antibody to Hexa Histidine (H6)

Specificity: The antibody is a mouse monoclonal antibody raised against H6. It has been selected for its ability to recognize H6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Various Species

Purification: Protein A + Protein G affinity chromatography

Immunogen: OVA Conjugated Hexa Histidine (H6)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for His Tag

Target: His Tag

Alternative Name: Hexa Histidine

Target Type: Tag

Background: 6xHis, His-Tag