Angiotensin I Converting Enzyme 1 antibody (AA 334-409)

Catalog No. ABIN7442318

The Rabbit Polyclonal anti-Angiotensin I Converting Enzyme 1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Angiotensin I Converting Enzyme 1 in samples from Mouse.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (AA 334-409) (ABIN7442318)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Binding Specificity: AA 334-409

Purpose: Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACE. It has been selected for its ability to recognize ACE in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Angiotensin I Converting Enzyme (ACE) corresdonding to Phe334~Leu409 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:160-650,Immunohistochemistry: 5-20 μg/mL,1:16-65,Immunocytochemistry: 5-20 μg/mL,1:16-65,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: Angiotensin I Converting Enzyme

Background: CD143, ACE1, DCP1, ACEI, ACE-I, Kininase II, Angiotensin-Converting Enzyme, Peptidyl-Dipeptidase A, Dipeptidyl Carboxypeptidase 1, Angiotensin-converting enzyme, soluble form

UniProt: P09470

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration