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IL-5 antibody (AA 20-134)

The Rabbit Polyclonal anti-IL-5 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect IL-5 in samples from Dog.
Catalog No. ABIN7430825

Quick Overview for IL-5 antibody (AA 20-134) (ABIN7430825)

Target

See all IL-5 (IL5) Antibodies
IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity

  • 74
  • 42
  • 21
  • 12
  • 8
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Dog

Host

  • 62
  • 31
  • 30
  • 2
  • 2
Rabbit

Clonality

  • 62
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  • 3
Polyclonal

Conjugate

  • 95
  • 14
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This IL-5 antibody is un-conjugated

Application

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  • 31
  • 19
  • 13
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  • 7
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 20
    • 15
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 20-134

    Purpose

    Polyclonal Antibody to Interleukin 5 (IL5)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against IL5. It has been selected for its ability to recognize IL5 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Interleukin 5 (IL5) corresdonding to Phe20~Ser134

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    IL-5 (IL5) (Interleukin 5 (IL5))

    Alternative Name

    Interleukin 5

    Background

    E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor,Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor

    UniProt

    Q95J76

    Pathways

    JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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