KIT antibody (AA 321-520)

Catalog No. ABIN7431107

This Rabbit Polyclonal antibody specifically detects KIT in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for KIT antibody (AA 321-520) (ABIN7431107)

Key Features:

• High quality KIT Primary Antibody for the detection of KIT.
• Reliable product with high standard validation data

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-KIT Antibody

Binding Specificity: AA 321-520

Purpose: Polyclonal Antibody to Stem Cell Factor Receptor (SCFR)

Specificity: The antibody is a rabbit polyclonal antibody raised against SCFR. It has been selected for its ability to recognize SCFR in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Stem Cell Factor Receptor (SCFR) corresdonding to Thr321~Pro520 (Accession # P10721) with N-terminal His Tag

Isotype: IgG

Product Specific Information:

What can the KIT Antibody ABIN7431107 be used for?: This polyclonal KIT Antibody detects KIT. The KIT Antibody has been validated for various applications and can be used for the detection of KIT and derivatives by Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

What validation data is available for this KIT Antibody?: The primary antibody has currently 6 product images that show its performance in a variety of applications. The product is currently available in 1 mL, 20 μL, 100 μL, 200 μL quantities. KIT Antibody for the detection of KIT and derivatives.

What is the function of KIT?: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: SCFR

Background: CD117, KIT, C-Kit, PBT, p145 c-Kit, C-Kit Receptor, Tyrosine-Protein Kinase, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, Piebald Trait

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity