ELANE antibody (AA 29-247)

Catalog No. ABIN7431272

This Rabbit Polyclonal antibody specifically detects ELANE in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for ELANE antibody (AA 29-247) (ABIN7431272)

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ELANE Antibody

Binding Specificity: AA 29-247

Purpose: Polyclonal Antibody to Neutrophil Elastase (NE)

Specificity: The antibody is a rabbit polyclonal antibody raised against NE. It has been selected for its ability to recognize NE in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Neutrophil Elastase (NE) corresdonding to Ile29~Arg247 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase

Background: ELANE, HLE, HNE, ELA2, PMN-E, Neutrophil Elastase, Medullasin, Polymorphonuclear Leukocyte Elastase, Bone Marrow Serine Protease, Leukocyte Elastase, PMN Elastase

UniProt: Q3UP87