CENPF antibody (AA 436-637)

Catalog No. ABIN7431644

This anti-CENPF antibody is a Rabbit Polyclonal antibody detecting CENPF in ELISA, WB, IHC (p), ICC and IHC (fro). Suitable for Human.

Quick Overview for CENPF antibody (AA 436-637) (ABIN7431644)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CENPF Antibody

Binding Specificity: AA 436-637

Purpose: Polyclonal Antibody to Centromere Protein F (CENPF)

Specificity: The antibody is a rabbit polyclonal antibody raised against CENPF. It has been selected for its ability to recognize CENPF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Centromere Protein F (CENPF) corresdonding to Gln436~Lys637 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF

Background: CENP-F, CENF, Hcp-1, Centromere protein F(350/400kD,Mitosin), AH antigen, Kinetochore protein CENPF, Mitosin

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection