Prohibitin antibody (AA 177-272)

Catalog No. ABIN7431794

This Rabbit Polyclonal antibody specifically detects Prohibitin in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Prohibitin antibody (AA 177-272) (ABIN7431794)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 177-272

Purpose: Polyclonal Antibody to Prohibitin (PHB)

Specificity: The antibody is a rabbit polyclonal antibody raised against PHB. It has been selected for its ability to recognize PHB in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Prohibitin (PHB) corresdonding to Lys177~Gln272 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling