GCK antibody (AA 3-226)

Catalog No. ABIN7429088

The Rabbit Polyclonal anti-GCK antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect GCK in samples from Mouse.

Quick Overview for GCK antibody (AA 3-226) (ABIN7429088)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GCK Antibody

Binding Specificity: AA 3-226

Purpose: Polyclonal Antibody to Glucokinase (GCK)

Specificity: The antibody is a rabbit polyclonal antibody raised against GCK. It has been selected for its ability to recognize GCK in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glucokinase (GCK) corresdonding to Asp3~Val226

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: Glucokinase

Background: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, Hexokinase-D, Hexokinase 4, Maturity Onset Diabetes Of The Young 2

UniProt: P52792

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process