Glypican 3 antibody (AA 343-559)

Catalog No. ABIN7432952

The Rabbit Polyclonal anti-Glypican 3 antibody has been validated for WB, ELISA, IHC (p), ICC and IHC (fro). It is suitable to detect Glypican 3 in samples from Human.

Quick Overview for Glypican 3 antibody (AA 343-559) (ABIN7432952)

Target: Glypican 3 (GPC3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Glypican 3 Antibody

Binding Specificity: AA 343-559

Purpose: Polyclonal Antibody to Glypican 3 (GPC3)

Specificity: The antibody is a rabbit polyclonal antibody raised against GPC3. It has been selected for its ability to recognize GPC3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glypican 3 (GPC3) corresdonding to Thr343~His559 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: DGSX, OCI5, SDYS, SGB, SGBS1, MXR7, Glypican Proteoglycan 3, GTR2-2, Intestinal protein OCI-5, Secreted glypican-3

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process