ACTN3 antibody (AA 162-416)

Catalog No. ABIN7444097

This anti-ACTN3 antibody is a Rabbit Polyclonal antibody detecting ACTN3 in WB, IHC, ICC and IP. Suitable for Human.

Quick Overview for ACTN3 antibody (AA 162-416) (ABIN7444097)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-ACTN3 Antibody

Binding Specificity: AA 162-416

Purpose: Polyclonal Antibody to Actinin Alpha 3 (ACTN3)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACTN3. It has been selected for its ability to recognize ACTN3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Actinin Alpha 3 (ACTN3) corresdonding to Glu162~Lys416 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000Immunohistochemistry: 5-20 μg/mL,1:25-100Immunocytochemistry: 5-20 μg/mL,1:25-100Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: ACTN3

Background: Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein

UniProt: Q08043

Pathways: Cell-Cell Junction Organization