JNK antibody (AA 138-382)

Catalog No. ABIN7439324

Product Details anti-JNK Antibody

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Binding Specificity: AA 138-382

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against JNK1. It has been selected for its ability to recognize JNK1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Cow, Dog, Goat, Guinea Pig, Human, Pig, Rat, Sheep

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant c-Jun N-terminal Kinase 1 (JNK1) corresdonding to Gly138~Val382 (Accession # Q91Y86)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK1 (MAPK8 Products)

Background: MAPK8, JNK1A2, JNK21B1/2, PRKM8, SAPK1, JUN N-Terminal Kinase, Stress-activated protein kinase 1c, Mitogen Activated Protein Kinase 8

UniProt: Q91Y86

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins