CD25 antibody (AA 22-236)

Catalog No. ABIN7440683

This anti-CD25 antibody is a Rabbit Polyclonal antibody detecting CD25 in WB, IHC, IP, ICC. Suitable for Mouse.

Quick Overview for CD25 antibody (AA 22-236) (ABIN7440683)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CD25 Antibody

Binding Specificity: AA 22-236

Purpose: Polyclonal Antibody to Interleukin 2 Receptor Alpha (IL2Ra)

Specificity: The antibody is a rabbit polyclonal antibody raised against IL2Ra. It has been selected for its ability to recognize IL2Ra in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Interleukin 2 Receptor Alpha (IL2Ra) corresdonding to Glu22~Lys236 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.51 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2Ra

Background: CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation