p300 antibody (AA 1-158)

Catalog No. ABIN7440794

The Rabbit Polyclonal anti-p300 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect p300 in samples from Rat.

Quick Overview for p300 antibody (AA 1-158) (ABIN7440794)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-p300 Antibody

Binding Specificity: AA 1-158

Purpose: Polyclonal Antibody to E1A Binding Protein P300 (EP300)

Specificity: The antibody is a rabbit polyclonal antibody raised against EP300. It has been selected for its ability to recognize EP300 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant E1A Binding Protein P300 (EP300) corresdonding to Met1~Pro158 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300

Background: KAT3B, p300, Histone Acetyltransferase p300

UniProt: Q91XT0

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size