CISH antibody (AA 1-258)

Catalog No. ABIN7444772

This anti-CISH antibody is a Rabbit Polyclonal antibody detecting CISH in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for CISH antibody (AA 1-258) (ABIN7444772)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CISH Antibody

Binding Specificity: AA 1-258

Purpose: Polyclonal Antibody to Cytokine Inducible SH2 Containing Protein (CISH)

Specificity: The antibody is a rabbit polyclonal antibody raised against CISH. It has been selected for its ability to recognize CISH in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cytokine Inducible SH2 Containing Protein (CISH) corresdonding to Met1~Leu258 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: CISH

Background: CIS, CIS-1, G18, SOCS, Suppressor of cytokine signaling

UniProt: Q9NSE2

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus