Phenylalanine Hydroxylase antibody (AA 228-452)

Catalog No. ABIN7438214

The Rabbit Polyclonal anti-Phenylalanine Hydroxylase antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Phenylalanine Hydroxylase in samples from Human.

Quick Overview for Phenylalanine Hydroxylase antibody (AA 228-452) (ABIN7438214)

Target: Phenylalanine Hydroxylase

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phenylalanine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Phenylalanine Hydroxylase Antibody

Binding Specificity: AA 228-452

Purpose: Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

Specificity: The antibody is a rabbit polyclonal antibody raised against PAH. It has been selected for its ability to recognize PAH in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phenylalanine Hydroxylase (PAH) corresdonding to Glu228~Lys452 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Background: PKU, PKU1, Phe-4-monooxygenase

UniProt: P00439