14-3-3 sigma/SFN antibody (AA 1-249)

Catalog No. ABIN7442123

This Rabbit Polyclonal antibody specifically detects 14-3-3 sigma/SFN in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 1-249) (ABIN7442123)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 1-249

Purpose: Polyclonal Antibody to Stratifin (SFN)

Specificity: The antibody is a rabbit polyclonal antibody raised against SFN. It has been selected for its ability to recognize SFN in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Stratifin (SFN) corresdonding to Met1~Ser249 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: Stratifin

Background: YWHAS, HME1, 14-3-3 Protein Sigma, Epithelial cell marker protein 1

UniProt: O70456

Pathways: p53 Signaling, Myometrial Relaxation and Contraction