PAK1 antibody (AA 252-501)

Catalog No. ABIN7442222

This anti-PAK1 antibody is a Rabbit Polyclonal antibody detecting PAK1 in WB and IHC. Suitable for Human.

Quick Overview for PAK1 antibody (AA 252-501) (ABIN7442222)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PAK1 Antibody

Binding Specificity: AA 252-501

Purpose: Polyclonal Antibody to p21 Protein Activated Kinase 1 (PAK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PAK1. It has been selected for its ability to recognize PAK1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant p21 Protein Activated Kinase 1 (PAK1) corresdonding to Glu252~Cys501 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: PAKalpha, p65-PAK, p21/Cdc42/Rac1-Activated Kinase 1, Serine/threonine-protein kinase PAK 1

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis