MPG antibody (AA 221-296)

Catalog No. ABIN7436513

The Rabbit Polyclonal anti-MPG antibody has been validated for WB, ELISA, IHC (p), ICC and IHC (fro). It is suitable to detect MPG in samples from Human.

Quick Overview for MPG antibody (AA 221-296) (ABIN7436513)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MPG Antibody

Binding Specificity: AA 221-296

Purpose: Polyclonal Antibody to N-Methylpurine DNA Glycosylase (MPG)

Specificity: The antibody is a rabbit polyclonal antibody raised against MPG. It has been selected for its ability to recognize MPG in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant N-Methylpurine DNA Glycosylase (MPG) corresdonding to Pro70~Lys220 and Leu221~Thr296 linked by a peptide LCQALA with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: N-Methylpurine DNA Glycosylase

Background: AAG, APNG, ADPG, CRA36.1, MDG, Mid1, PIG11, PIG16, Anpg, DNA-3-Methyladenine Glycosylase, Alkyladenine DNA Glycosylase, 3-alkyladenine DNA glycosylase

UniProt: P29372

Pathways: DNA Damage Repair