PDX1 antibody (AA 1-284)

Catalog No. ABIN7441508

The Rabbit Polyclonal anti-PDX1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PDX1 in samples from Mouse.

Quick Overview for PDX1 antibody (AA 1-284) (ABIN7441508)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDX1 Antibody

Binding Specificity: AA 1-284

Purpose: Polyclonal Antibody to Insulin Promoter Factor 1 (IPF)

Specificity: The antibody is a rabbit polyclonal antibody raised against IPF. It has been selected for its ability to recognize IPF in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Insulin Promoter Factor 1 (IPF) corresdonding to Met1~Arg284 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: Insulin Promoter Factor 1

Background: IDX1, STF1, MODY4, PDX1, GSF, IUF1, Pancreatic And Duodenal Homeobox 1, Somatostatin Transcription Factor 1, Glucose-sensitive factor, Insulin upstream factor 1

UniProt: P52946

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location