FBXW7 antibody (AA 279-515)

Catalog No. ABIN7443293

This anti-FBXW7 antibody is a Rabbit Polyclonal antibody detecting FBXW7 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for FBXW7 antibody (AA 279-515) (ABIN7443293)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-FBXW7 Antibody

Binding Specificity: AA 279-515

Purpose: Polyclonal Antibody to F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

Specificity: The antibody is a rabbit polyclonal antibody raised against FBXW7. It has been selected for its ability to recognize FBXW7 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) corresdonding to Asp279~Val515 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blottin:g: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7

Background: AGO, CDC4, FBW7, FBX30, FBXW6, SEL-10, SEL10, Archipelago Homolog, F-box and WD-40 domain-containing protein 7

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway