eIF4EBP1 antibody (AA 2-118)

Catalog No. ABIN7436819

Product Details anti-eIF4EBP1 Antibody

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Binding Specificity: AA 2-118

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EIF4EBP1. It has been selected for its ability to recognize EIF4EBP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) corresdonding to Ser2-Ile118 (Accession # Q13541) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:200-800 Immunohistochemistry: 5-20 μg/mL

1:20-80 Immunocytochemistry: 5-20 μg/mL

1:20-80 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1 Products)

Background: 4EBP1, BP-1, PHAS-I, Phosphorylated Heat And Acid-Stable Protein Regulated By Insulin 1

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling