GCK antibody

Catalog No. ABIN7234070

The Rabbit Polyclonal anti-GCK antibody (ABIN7234070) specifically detects GCK in WB, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for GCK antibody (ABIN7234070)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GCK Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen Affinity Purification

Immunogen: Recombinant Mouse Glucokinase protein

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000 IHC 1:100-1:200 IF 1:100-1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.05 % Proclin300 and 50 % glycerol, pH 7.4.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Molecular Weight:

Observed_MW: 52 kDa

Calculated_MW: 52 kDa

UniProt: P52792

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process