ERCC6L antibody

Catalog No. ABIN7236887

The Rabbit Polyclonal anti-ERCC6L antibody has been validated for ELISA and IHC. It is suitable to detect ERCC6L in samples from Human.

Quick Overview for ERCC6L antibody (ABIN7236887)

Target: ERCC6L (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like (ERCC6L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC6L antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-ERCC6L Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human ERCC6L

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ERCC6L

Target: ERCC6L (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like (ERCC6L))

Alternative Name: ERCC6L

Background: PICH (Plk1-interacting checkpoint helicase), also known as DNA excision repair protein ERCC-6-like (ERCC6L) or tumor antigen BJ-HCC-15, is a 1,250 amino acid protein belonging to the SNF2/RAD54 helicase family. PICH is a DNA helicase and an essential component of the spindle assembly checkpoint. During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin. PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores. PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region. PICH/Plk interaction is also required for correct Plk localization to the kinetochore. PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.

UniProt: Q2NKX8