SPI1 antibody

Catalog No. ABIN7251703

This Rabbit Polyclonal antibody specifically detects SPI1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPI1 antibody (ABIN7251703)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SPI1 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human SPI1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.SPI1 (Spi-1 Proto-Oncogene) is a Protein Coding gene. Diseases associated with SPI1 include Inflammatory Diarrhea and Interdigitating Dendritic Cell Sarcoma. Among its related pathways are Validated targets of C-MYC transcriptional repression and C-MYB transcription factor network. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and RNA binding. An important paralog of this gene is SPIB.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 31 kDa

UniProt: P17947

Pathways: Stem Cell Maintenance