EIF5B antibody

Catalog No. ABIN7258398

The Rabbit Polyclonal anti-EIF5B antibody has been validated for WB and IF. It is suitable to detect EIF5B in samples from Human, Mouse and Rat.

Quick Overview for EIF5B antibody (ABIN7258398)

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-EIF5B Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human EIF5B (NP_056988.3).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Alternative Name: EIF5B

Background: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Molecular Weight:

Observed_MW: 179 kDa

Calculated_MW: 138 kDa

Gene ID: 9669

UniProt: O60841