PIKFYVE antibody

Catalog No. ABIN7011299

This anti-PIKFYVE antibody is a Rabbit Polyclonal antibody detecting PIKFYVE in WB, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for PIKFYVE antibody (ABIN7011299)

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIKFYVE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PIKFYVE Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human PIKFYVE (NP_689884.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:100 IF 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PIKFYVE

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Alternative Name: PIKFYVE

Background: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve, also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD), an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight:

Observed_MW: 280 kDa

Calculated_MW: 50 kDa/51 kDa/61 kDa/237 kDa

Gene ID: 200576

UniProt: Q9Y2I7

Pathways: Inositol Metabolic Process