PIKFYVE antibody

Catalog No. ABIN7581220

The Rabbit Polyclonal anti-PIKFYVE antibody (ABIN7581220) specifically detects PIKFYVE in WB, ICC and IP. The antibody is reactive with Various Species samples.

Quick Overview for PIKFYVE antibody (ABIN7581220)

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIKFYVE antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PIKFYVE Antibody

Purpose: Rabbit polyclonal antibody to murine PIKfyve with reactivity to mouse/rat and human PIKfyve

Specificity: The PIKfyve antibody is directed at an N-terminal region of PIKfyve and detects mammalian forms of PIKfyve with an expected molecular weight of approximately 237 kDa.

Purification: affinity purified using Protein A chromatography

Immunogen: synthetic fusion peptide of aa 1-100

Isotype: IgG

Application Details

Application Notes: IF/ICC – 2-5 ug/mL Western – 1.0 ug/mL Immunoprecipitation – 1.0 ug/mL Other in vitro and cellular applications are possible using this antibody, but have not been verified.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: protect from direct light and store at 4°C for up to 30 days. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Target Details for PIKFYVE

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Alternative Name: PIKfyve

Background: PIKfyve (aka PIP5K3) is an approximately 237 kDa lipid kinase enzyme. It is primarily found on early endosomes where it synthesizes PI(3,5)P2 from membrane-associated PI(3)P. Endosomal PIKfyve forms a complex with ArPIKfyve (Vac14) and Sac3 to regulate fusion, transport, and export of endosomes in the cell. PIKfyve also has protein kinase activity in the form of self-regulatory autophosphorylation.

Pathways: Inositol Metabolic Process