Aurora A antibody (pThr198, pThr232, pThr288)

Catalog No. ABIN7265292

The Rabbit Polyclonal anti-Aurora A antibody has been validated for WB. It is suitable to detect Aurora A in samples from Human and Rat.

Quick Overview for Aurora A antibody (pThr198, pThr232, pThr288) (ABIN7265292)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aurora A Antibody

Binding Specificity: pThr198, pThr232, pThr288

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding T288/T232/T198 of human AURKA/AURKB/AURKC.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background: The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene./This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. /This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.

Molecular Weight: 35K kDa/48 kDa

Gene ID: 6790, 9212, 6795

UniProt: O14965, Q96GD4, Q9UQB9

Pathways: Cell Division Cycle, Asymmetric Protein Localization