DDX4 antibody (AA 601-700)

Catalog No. ABIN701365

This Rabbit Polyclonal antibody specifically detects DDX4 in WB, FACS, IHC (p), IF (p) and IHC (fro). It exhibits reactivity toward Chicken and has been mentioned in 2+ publications.

Quick Overview for DDX4 antibody (AA 601-700) (ABIN701365)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DDX4 Antibody

Binding Specificity: AA 601-700

Cross-Reactivity: Chicken

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DDX4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DDX4 antibody (ABIN701365)

Shirzeyli, Tayyebiazar, Aliakbari, Ghasemi, Eini, Shirzeyli, Vanaki, Sobhani: "Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells." in: , Vol. 17, Issue 2, pp. 123-133, (2022) (PubMed).

Imus, Roe, Charter, Durrant, Jensen: "Transfer and detection of freshly isolated or cultured chicken (Gallus gallus) and exotic species' embryonic gonadal germ stem cells in host embryos." in: Zoological science, Vol. 31, Issue 6, pp. 360-8, (2014) (PubMed).

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4

Background:

Synonyms: VASA, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4

Background: Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene ID: 54514

UniProt: Q9NQI0