CLEC9A antibody (PE)

Catalog No. ABIN7013960

The PE-conjugated Mouse Monoclonal anti-CLEC9A antibody (Clone 8F9) (ABIN7013960) specifically detects CLEC9A in FACS. The antibody is reactive with Human samples.

Quick Overview for CLEC9A antibody (PE) (ABIN7013960)

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLEC9A antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 8F9

Product Details anti-CLEC9A Antibody (PE)

Purpose: Anti-Hu CD370 PE

Characteristics: The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: RBL-2H3 cells expressing human CLEC9A fused to an HA epitope

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CLEC9A

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Alternative Name: CD370

Background: C-type lectin domain containing 9A,CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.,CLEC9A, DNGR1, DNGR-1, UNQ9341

Gene ID: 283420

UniProt: Q6UXN8