SIRPA antibody

Catalog No. ABIN7013993

The Mouse Monoclonal anti-SIRPA antibody (Clone 15-414) (ABIN7013993) specifically detects SIRPA in FACS. The antibody is reactive with Human samples.

Quick Overview for SIRPA antibody (ABIN7013993)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 15-414

Product Details anti-SIRPA Antibody

Purpose: Anti-Hu CD172a Purified

Characteristics: The mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons.

Purification: Purified by protein-A affinity chromatography.

Immunogen: Kg-1a cell line

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background: Signal regulatory protein alpha,CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.,PTPNS1, BIT, MFR, SIRPA, SHPS1

Gene ID: 140885

UniProt: P78324