KEAP1 antibody (AA 65-160) (HRP)

Catalog No. ABIN702094

This Rabbit Polyclonal antibody specifically detects KEAP1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Pig and Rabbit.

Quick Overview for KEAP1 antibody (AA 65-160) (HRP) (ABIN702094)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-KEAP1 Antibody (HRP)

Binding Specificity: AA 65-160

Cross-Reactivity: Human, Mouse, Pig, Rabbit, Rat

Predicted Reactivity: Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KEAP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background:

Synonyms: INrf2, KLHL19, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1, KIAA0132

Background: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location