ATP1B2 antibody (Extracellular)

Catalog No. ABIN7042952

Product Details anti-ATP1B2 Antibody

Target: ATP1B2 (ATPase, Na+/K+ Transporting, beta 2 Polypeptide (ATP1B2))

Binding Specificity: AA 99-112, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Beta 2 Na+/K+ ATPase

Specificity: Extracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - identical, mouse 13,14 amino acid residues identical

Characteristics: Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (ABIN7042952, ABIN7044643 and ABIN7044644) is a highly specific antibody directed against an epitope of the rat ATP1B2. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize ATP1B2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DTESWDQHVQKLNK, corresponding to amino acid residues 99-112 of rat ATP1B2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IHC

Negative Control: (ABIN7234809)

Blocking Peptide: (ABIN7234809)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP1B2

Target: ATP1B2 (ATPase, Na+/K+ Transporting, beta 2 Polypeptide (ATP1B2))

Alternative Name: ATP1B2 (ATP1B2 Products)

Background: ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG,P-type ATPases are a large family of molecular pumps that exploit a phosphorylated enzyme intermediate in a two-step mechanism of ATP hydrolysis, cycling through states which are associated with ion transport or ion counter-transport. The Na+/K+-ATPase, a member of this family, is almost exclusively found in animals, although close homologues have been reported in certain archaea, algae and oomycetes.The Na+/K+-ATPase is comprised of a nucleotide-binding (N) and phosphorylation (P) domain, a transmembrane core (M1-M6) and a large carboxy-terminal M7-M10 segment. Na+/K+-ATPase undergoes large conformational changes as part of its functional cycle giving rise to two distinct enzymatic states: E1, which is a high-affinity state for the primary transported ion- Na+ and E2, which is the low-affinity state for the Na+ ion. The two states arise from the autocatalysed formation and breakdown of a phosphoenzyme intermediate, coupled to the binding, occlusion, translocation and release of ions. The phosphorylation site is the Asp residue of a conserved DKTGT motif. The core of the membrane transport domain encompasses transmembrane helices M1-M6, which hold the main ion-binding sites and are necessary for cytoplasmic and extracellular ion transport. A terminal R domain extension, which serves as a regulatory unit, can be found in the C-terminal of the protein. This unit is auto-inhibitory and is predicted to restrict transmembrane helix movements and/or access of ions to the membrane transport core1. Na+/K+-ATPase has been implicated in the pathogenesis of Alzheimer's Disease. A deficiency in several Na+/K+-ATPase isoform genes induce learning and memory deficits, and the α isoform is altered in Alzheimer's Disease2.

Alternative names: Beta 2 Na+/K+ ATPase, ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

Gene ID: 24214

NCBI Accession: NM_001678

UniProt: P13638

Pathways: Thyroid Hormone Synthesis