CXCR7 antibody (Extracellular, N-Term)

Catalog No. ABIN7886252

The Rabbit Polyclonal anti-CXCR7 antibody (ABIN7886252) specifically detects CXCR7 in WB, FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for CXCR7 antibody (Extracellular, N-Term) (ABIN7886252)

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CXCR7 Antibody

Binding Specificity: AA 2-16, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse -14,15 amino acid residues identical, human - 12

Characteristics: Anti-CXCR7 (ACKR3) (extracellular) Antibody (ABIN7043043, ABIN7044181 and ABIN7044182) is a highly specific antibody directed against an epitope of the rat CXCR7 chemokine receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR7 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVHLFDYVEPGNYSD, corresponding to amino acid residues 2-16 of rat CXCR7 (ACKR3)

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235067)

Blocking Peptide: (ABIN7235067)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Alternative Name: ACKR3

Background:

Synonyms: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159

Description: Chemokines are molecules that control the migration and positioning of the immune system cells. In addition to their function as chemo-attractants they have various other roles in the maturation of immune cells such as T-cell development in the thymus and homeostatic retention of immune cells in the bone marrow1.Chemokines bind to chemokine receptors, members of the G-protein coupled receptor superfamily. They are comprised of seven transmembrane receptors with extracellular N-terminus, three extracellular and three intracellular loops and an intracellular C-terminus. One of the intracellular domains of the receptor couples with G proteins and begins a cascade of signal transduction2.Until recently the CXCR-7 was an "orphan" receptor with its endogenous ligand unknown. This receptor is encoded by the CXCR7/RDC-1 gene which was mapped to mouse chromosome 1 and human chromosome 2. Homology has been shown with the viral gene ORF74 suggesting the receptor might signal without the binding of a ligand. Currently it is known that the chemokines CXCL12 and CXCL11 bind to CXCR-73.CXCR-7 is tightly linked to the regulation of B cell development and differentiation indicating it might have a role in the formation of B memory cells4. CXCR-7 was also found to be associated with tumors where its levels are elevated in many tumor cell lines such as endothelial cells, fetal liver cells and placenta cells5. Despite this suggested role, there is growing evidence that this receptor does not function as a regular GPCR6.

Gene ID: 84348

NCBI Accession: NM_020311

UniProt: O89039

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling