Chloride Channel 5 antibody (Extracellular)

Catalog No. ABIN7043053

Product Details anti-Chloride Channel 5 Antibody

Target: Chloride Channel 5 (CLCN5)

Binding Specificity: AA 401-415, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chloride Channel 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to CLC-5 (CLCN5) Channel

Specificity: Extracellular

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti- Antibody (ABIN7043053 and ABIN7044124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLCN5 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235090)

Blocking Peptide: (ABIN7235090)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Chloride Channel 5

Target: Chloride Channel 5 (CLCN5)

Alternative Name: CLCN5 (CLCN5 Products)

Background: CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS,CLC5 is a member of the voltage-dependent Cl- channel family that includes nine known members. Based on sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits. CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes, where it is believed to contribute to their acidification.The channel is mainly expressed in the kidney, although it has also been found in the small intestine, brain and liver.Mutations in the CLC5 gene cause Dent's disease, an X-linked renal disorder.

Alternative names: CLC-5, CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS

Gene ID: 25749

NCBI Accession: NM_000084

UniProt: P51796